Using CRISPR to Treat Cystic Fibrosis

In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

• Group Size: For 8 Lab Groups
• Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended
• Time Required: Complete in 45 minutes

The kit includes:

• Instructions,
• Ready-to-Load QuickStrip™ DNA Samples
• UltraSpec-Agarose™
• Electrophoresis Buffer (50X)
• 10X Gel Loading Solution,
• FlashBlue™ DNA Stain

Additional required equipment & materials (not supplied with the kit):

• DNA Electrophoresis tank and accessories including casting trays and special power supply
• Micropipettes: 5-50 µl (Optional)
• Balance
• White Light Box
• Microwave or Hot Plate

*PLEASE NOTE: This kit has been developed for being suitable for use in education only and is not for diagnostic purposes*