Simulation Of Hiv Detection

The HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient’s blood and adding them to a microtiter plate coated with HIV antigen. If HIV antibodies are present in the blood, they will bind to the antigens on the plate. This binding is detected with an enzyme-linked secondary antibody that causes a colour change upon addition of substrate.

In this experiment, your students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

• Group Size: for 10 lab groups
• Storage: Refrigerator
• Time Required: 60 minutes

The kit includes:

• Full instructions
• 10X ELISA wash buffer
• ELISA dilution buffer
• Antigen (lypholized)
• Primary antibody (lypholized)
• Secondary antibody (lypholized)
• ABTS (lypholized)
• ABTS reaction buffer
• Microtitre plates
• Transfer pipettes
• Snap-top microcentrifuge tubes
• 15ml conical tubes

Additional required materials (not included with the kit):

• Distilled water
• Beakers
• Disposable laboratory gloves
• Automatic Micropipette & tips (recommended)
• Incubator (B8A64397)