Human Dna Typing Using Pcr

This Human DNA Typing Kit allows your students to use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype!

They can then compare their class results with others around the world over the internet.

• Group Size: for 25 students
• Storage: Some components require freezer storage on receipt
• Time Required: Extraction – 50 minutes, PCR set-up – 10 minutes, PCR – 90 minutes, Electrophoresis – 60 minutes, staining – 5 minutes to overnight

The kit includes:

• Full Instructions
• PCR EdvoBeads™ PLUS
• PV92 LyphoPrimer™ Mix
• EdvoQuick™ DNA Ladder
• LyphoControl™
• Universal DNA Buffer
• TE Buffer
• Proteinase K
• UltraSpec-Agarose™
• TBE Electrophoresis Buffer Powder
• SYBR® Safe Stain
• FlashBlue™ Stain
• Microcentrifuge & PCR Tubes
• Disposable Plastic Cups
• Salt Packets
• a 15 mL conical tube.

Additional required materials (not included with the kit):

• DNA Electrophoresis Tank, with accessories including casting trays, micropipettes & special power supply (B8R03273)
• Thermal Cycler (B8R07450)
• UV or Blue Light Transilluminator 
• White Light Box – optional (B8R07358)
• 2 x Water Bath (B8R06048)
• Microcentrifuge (B8R04574)
• Microwave or Hot plate